8ACX

Pathogen effector of Zymoseptoria tritici: Zt-KP4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-03
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	109.108, 109.108, 252.755
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.440 - 1.900
R-factor	0.1715
Rwork	0.171
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Itasser model
RMSD bond length	0.014
RMSD bond angle	1.889
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.440	2.010
High resolution limit [Å]	1.900	1.900
Number of reflections	86240	2800
<I/σ(I)>	14.9
Completeness [%]	97.0
Redundancy	9.25
CC(1/2)	0.999	0.381

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	sodium acetate and PEG 300