8ACU
Structure of Bacillus subtilis Rel in complex with DarB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.401, 98.141, 126.775 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.720 - 2.970 |
R-factor | 0.2627 |
Rwork | 0.260 |
R-free | 0.31530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6s2t |
RMSD bond length | 0.002 |
RMSD bond angle | 0.511 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.600 | 3.080 |
High resolution limit [Å] | 2.970 | 2.970 |
Rpim | 0.061 | 0.851 |
Number of reflections | 22489 | 1023 |
<I/σ(I)> | 11.1 | |
Completeness [%] | 95.0 | |
Redundancy | 20.1 | |
CC(1/2) | 0.998 | 0.387 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.16 | 0.1 M Magnesium chloride hexahydrate 0.1 M Sodium HEPES 7.5 10 % w/v PEG 4000 |