8ACM

Crystal structure of WT p38alpha

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979257
Spacegroup name	P 21 21 21
Unit cell lengths	65.320, 75.160, 78.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.345 - 2.140
Rwork	0.201
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4loo
RMSD bond length	0.005
RMSD bond angle	1.294
Data reduction software	iMOSFLM (7.2.2)
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.670	2.260
High resolution limit [Å]	2.140	2.140
Rmerge	0.085	0.380
Number of reflections	20793	2973
<I/σ(I)>	10.6	3.2
Completeness [%]	94.3	93.9
Redundancy	5.5	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1,M HEPES pH 7.5, 25,%w/v PEG 3350