8AC8
The nucleoprotein complex of Rep protein with DUE ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979310 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 47.400, 85.310, 146.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.850 - 1.600 |
R-factor | 0.2129 |
Rwork | 0.212 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rep |
RMSD bond length | 0.006 |
RMSD bond angle | 0.828 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.850 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 79263 | 12422 |
<I/σ(I)> | 17.92 | |
Completeness [%] | 99.6 | |
Redundancy | 12.99 | |
CC(1/2) | 0.990 | 0.431 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | PEG 8000, ethylene glycol, HEPES, halogens |