8ABQ
Structure of the SNX1-SNX5 complex, Pt derivative
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.07158 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 52.690, 51.470, 192.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.470 - 2.810 |
R-factor | 0.2571 |
Rwork | 0.252 |
R-free | 0.30550 |
Structure solution method | MAD |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.18-3855-000) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 192.319 | 51.469 | 2.950 |
High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
Rmerge | 0.033 | 0.991 | |
Rmeas | 0.095 | 0.060 | 1.182 |
Rpim | 0.038 | 0.027 | 0.469 |
Total number of observations | 155315 | 4905 | 22205 |
Number of reflections | 24815 | 840 | 3609 |
<I/σ(I)> | 10.3 | 31 | 1.7 |
Completeness [%] | 96.2 | 95.6 | 96.1 |
Redundancy | 6.3 | 5.8 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 11% PVA, 10% 1-propanol, 100mM HEPES pH 7.5 |