8A9O
Structure of the polyamine acetyltransferase DpA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98015 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.330, 69.295, 55.773 |
| Unit cell angles | 90.00, 109.08, 90.00 |
Refinement procedure
| Resolution | 21.860 - 1.587 |
| R-factor | 0.1906 |
| Rwork | 0.189 |
| R-free | 0.21990 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.300 | 1.593 |
| High resolution limit [Å] | 1.587 | 1.587 |
| Rpim | 0.033 | 0.394 |
| Number of reflections | 37959 | 2163 |
| <I/σ(I)> | 14.55 | |
| Completeness [%] | 90.1 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.996 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.16 | 0.2M Sodium acetate trihydrate, 0.1M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000 |
