8A9D

Multicrystal room temperature structure of Lysozyme collected using a double multilayer monochromator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE VMXi
Synchrotron site	Diamond
Beamline	VMXi
Temperature [K]	293
Detector technology	PIXEL
Collection date	2022-05-09
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.7749
Spacegroup name	P 43 21 2
Unit cell lengths	78.485, 78.485, 38.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.500 - 2.100
R-factor	0.199
Rwork	0.194
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2lyz
RMSD bond length	0.002
RMSD bond angle	0.361
Data reduction software	xia2.multiplex
Data scaling software	xia2.multiplex
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.490	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.524	3.260
Rmeas	0.543	3.369
Rpim	0.137	0.834
Number of reflections	7390	371
<I/σ(I)>	6.2	1.2
Completeness [%]	100.0	100
Redundancy	15.2	15.99
CC(1/2)	1.000	0.299

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.5	293	1M NaCl, 0.1 Na Acetate pH 4.5, 30% (v/v) PEG 400.