8A9C
Structure of truncated 1-deoxy-D-xylulose 5-phosphate synthase (DXS) from Klebsiella pneumoniae in complex with cofactor TPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-19 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.673, 72.559, 91.438 |
| Unit cell angles | 90.00, 108.46, 90.00 |
Refinement procedure
| Resolution | 43.370 - 1.800 |
| R-factor | 0.1561 |
| Rwork | 0.154 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o1s |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.253 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.370 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.561 |
| Number of reflections | 100675 | 14606 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 97.9 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 3000, HEPES |
