8A90

Crystal structure of FrsH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999987
Spacegroup name	P 41 21 2
Unit cell lengths	117.715, 117.715, 234.261
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.020 - 2.574
R-factor	0.2185
Rwork	0.217
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jo0
RMSD bond length	0.004
RMSD bond angle	0.882
Data reduction software	XDS (1.20_4444)
Phasing software	PHENIX (1.20_4444)
Refinement software	PHENIX (1.20_4444)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.020	2.665
High resolution limit [Å]	2.574	2.574
Rmerge	0.151
Number of reflections	87244	4482
<I/σ(I)>	7.22	1.01
Completeness [%]	93.0
Redundancy	4.2
CC(1/2)	0.991	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.10 M HEPES pH 7.8, 10.89% (w/v) PEG 20000, 0.08 M KAc, 1.36% glycerol