8A8X
TRIM7 PRYSPRY in complex with a MNV1-NS3 peptide HDDFGLQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-04-14 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.252, 53.668, 73.595 |
| Unit cell angles | 90.00, 105.55, 90.00 |
Refinement procedure
| Resolution | 28.337 - 2.370 |
| Rwork | 0.189 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8a5l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.653 |
| Data reduction software | xia2 (FastDP) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.910 | 2.430 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Number of reflections | 15418 | 712 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 97.4 | |
| Redundancy | 3.3 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291.15 | 1 M DL-Malic Acid pH 5, 0.1 M BIS-TRIS Propane pH 6.3 |
