8A8S

30S ribosomal protein S24e from Thermococcus kodakarensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-01
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.69
Spacegroup name	P 1 21 1
Unit cell lengths	23.551, 55.272, 37.579
Unit cell angles	90.00, 98.98, 90.00

Refinement procedure

Resolution	30.810 - 1.659
Rwork	0.220
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v94
Data reduction software	XDS (2020417)
Data scaling software	Aimless (0.74)
Phasing software	PHASER
Refinement software	PHENIX (V1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.100	1.687
High resolution limit [Å]	1.659	1.659
Rmerge		0.320
Number of reflections	9962	453
<I/σ(I)>	10.5
Completeness [%]	88.0
Redundancy	1.6
CC(1/2)	0.997	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0,1M sodium acetate pH 4,6 30% PEG 300