8A8F
Crystal structure of Glc7 phosphatase in complex with the regulatory region of Ref2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-16 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 90.230, 90.230, 101.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.120 - 1.850 |
| R-factor | 0.1935 |
| Rwork | 0.192 |
| R-free | 0.22050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7qwj |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.120 | 1.917 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.047 | 1.224 |
| Number of reflections | 41059 | 4028 |
| <I/σ(I)> | 20.46 | 1.84 |
| Completeness [%] | 100.0 | 98.82 |
| Redundancy | 10 | 10 |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 40 % (v/v) 1,4-Butanediol and 0.1 M Tris-HCl pH 8.5 |
