8A8F

Crystal structure of Glc7 phosphatase in complex with the regulatory region of Ref2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	90.230, 90.230, 101.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.120 - 1.850
R-factor	0.1935
Rwork	0.192
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7qwj
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.120	1.917
High resolution limit [Å]	1.850	1.850
Rmerge	0.047	1.224
Number of reflections	41059	4028
<I/σ(I)>	20.46	1.84
Completeness [%]	100.0	98.82
Redundancy	10	10
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	40 % (v/v) 1,4-Butanediol and 0.1 M Tris-HCl pH 8.5