8A7V
Room temperature rsEGFP2 in its OFF-state obtained with SFX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SACLA BEAMLINE BL3 |
Synchrotron site | SACLA |
Beamline | BL3 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2019-11-08 |
Detector | MPCCD |
Wavelength(s) | 1.18 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.970, 63.040, 72.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.440 - 1.460 |
R-factor | 0.1709 |
Rwork | 0.170 |
R-free | 0.19520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6t39 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.281 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.440 | 1.514 |
High resolution limit [Å] | 1.460 | 1.463 |
Number of reflections | 36821 | 340 |
<I/σ(I)> | 7.537 | |
Completeness [%] | 88.8 | |
Redundancy | 191.82 | |
CC(1/2) | 0.980 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 120 mM HEPES pH 8.0, 1.5 M ammonium sulphate, 20 mM NaCl |