8A7V
Room temperature rsEGFP2 in its OFF-state obtained with SFX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2019-11-08 |
| Detector | MPCCD |
| Wavelength(s) | 1.18 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.970, 63.040, 72.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.440 - 1.460 |
| R-factor | 0.1709 |
| Rwork | 0.170 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6t39 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.281 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.440 | 1.514 |
| High resolution limit [Å] | 1.460 | 1.463 |
| Number of reflections | 36821 | 340 |
| <I/σ(I)> | 7.537 | |
| Completeness [%] | 88.8 | |
| Redundancy | 191.82 | |
| CC(1/2) | 0.980 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 120 mM HEPES pH 8.0, 1.5 M ammonium sulphate, 20 mM NaCl |
