8A6Q
Room temperature rsEGFP2 with a chlorinated chromophore 5 ps after photoexcitation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2021-06-19 |
| Detector | MPCCD |
| Wavelength(s) | 1.18 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.990, 62.910, 72.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.790 - 1.630 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.20710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6pft |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.460 | 1.688 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 30206 | 2952 |
| <I/σ(I)> | 5.512 | |
| Completeness [%] | 100.0 | |
| Redundancy | 264.9 | |
| CC(1/2) | 0.980 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 1.3M ammonium sulfate, 100mM Hepes pH 8.1, 20mM NaCl |
