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8A6Q

Room temperature rsEGFP2 with a chlorinated chromophore 5 ps after photoexcitation

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeFREE ELECTRON LASER
Source detailsSACLA BEAMLINE BL3
Synchrotron siteSACLA
BeamlineBL3
Temperature [K]293
Detector technologyCCD
Collection date2021-06-19
DetectorMPCCD
Wavelength(s)1.18
Spacegroup nameP 21 21 21
Unit cell lengths51.990, 62.910, 72.030
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution22.790 - 1.630
R-factor0.1735
Rwork0.172
R-free0.20710
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)6pft
Data reduction softwareCrystFEL
Data scaling softwareCrystFEL
Phasing softwarePHENIX
Refinement softwarePHENIX (1.15.2_3472)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]31.4601.688
High resolution limit [Å]1.6301.630
Number of reflections302062952
<I/σ(I)>5.512
Completeness [%]100.0
Redundancy264.9
CC(1/2)0.9800.520
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1MICROBATCH2931.3M ammonium sulfate, 100mM Hepes pH 8.1, 20mM NaCl

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