8A60
Crystal structure of FhuA in complex with the superinfection exclusion lipoprotein Llp
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-12 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 98.287, 98.287, 236.588 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.850 - 3.370 |
| R-factor | 0.2558 |
| Rwork | 0.253 |
| R-free | 0.30740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1by3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.553 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20rc3_4406: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.110 | 3.610 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Number of reflections | 15120 | 697 |
| <I/σ(I)> | 9.2 | 1.7 |
| Completeness [%] | 87.4 | 48 |
| Redundancy | 14.4 | |
| CC(1/2) | 0.990 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 12-16% PEG 4000 0.4 M sodium isothiocyanate 0.1 M sodium acetate |
