8A5M

TRIM7 PRYSPRY in complex with a MNV1-NS6 peptide LEALEFQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-04-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	40.919, 112.972, 53.036
Unit cell angles	90.00, 102.93, 90.00

Refinement procedure

Resolution	56.550 - 2.918
Rwork	0.193
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ow2
RMSD bond length	0.006
RMSD bond angle	1.537
Data reduction software	xia2 (dials)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.970	3.100
High resolution limit [Å]	2.918	2.920
Number of reflections	10246	524
<I/σ(I)>	24.4
Completeness [%]	99.9
Redundancy	3.5
CC(1/2)	0.960	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291.15	1 M Sodium Potassium Phosphate pH 5