8A5L

TRIM7 PRYSPRY in complex with a 2BC peptide TIEALFQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-08-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 65
Unit cell lengths	79.887, 79.887, 53.205
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.018 - 1.622
Rwork	0.156
R-free	0.18840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ow2
RMSD bond length	0.012
RMSD bond angle	1.704
Data reduction software	xia2 (FastDP)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	1.660
High resolution limit [Å]	1.620	1.620
Number of reflections	24565	1780
<I/σ(I)>	11.1
Completeness [%]	99.7
Redundancy	4.9
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	291.15	1 M Sodium Potassium Tartrate, 0.1 M HEPES pH 6.8 (adjusted with HCl)