8A59

C-type lectin-like domain (CTLD) and Sushi-like domain of human CD93

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	63.712, 112.504, 73.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.500 - 1.920
R-factor	0.212
Rwork	0.209
R-free	0.26660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-Q9NPY3-F1
RMSD bond length	0.008
RMSD bond angle	1.569
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.260	2.020
High resolution limit [Å]	1.920	1.920
Rmerge	0.073	1.226
Rmeas	0.076	1.278
Rpim	0.022	0.356
Number of reflections	20515	2956
<I/σ(I)>	12.3	2.2
Completeness [%]	100.0	100
Redundancy	12.3	12.7
CC(1/2)	0.999	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M Ammonium sulfate, 1 M Lithium sulfate and 0.1 M Sodium citrate