8A4J

Human GDAP1, A247V mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	73.288, 115.688, 115.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.280 - 2.680
R-factor	0.2541
Rwork	0.252
R-free	0.28380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7alm
RMSD bond length	0.015
RMSD bond angle	1.449
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.840
High resolution limit [Å]	2.680	2.680
Rmerge	0.088	1.627
Rmeas	0.091	169.000
Number of reflections	28237	4457
<I/σ(I)>	16.9	1.88
Completeness [%]	99.7	99.9
Redundancy	13.1	13.5
CC(1/2)	0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	277	0.15 M DL-malic acid (pH 7.3), 20% (w/v) PEG3500