8A4E

Room temperature structure of AtPhot2LOV2 in a photostationary equilibrium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	293
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 43 21 2
Unit cell lengths	41.555, 41.555, 132.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.230 - 1.960
R-factor	0.1889
Rwork	0.187
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qqk
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.660	2.080
High resolution limit [Å]	1.960	1.960
Rpim	0.082	0.564
Number of reflections	7757	3612
<I/σ(I)>	5.7	1.5
Completeness [%]	91.9
Redundancy	8.4
CC(1/2)	0.980	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM MES pH 6.0, 4 to 9 % PEG8000, and 50 to 200 mM calcium acetate