8A4B
Structure of human Rep15:Rab3B_Q81L complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977930 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.840, 107.960, 91.100 |
| Unit cell angles | 90.00, 106.47, 90.00 |
Refinement procedure
| Resolution | 49.540 - 2.800 |
| R-factor | 0.2254 |
| Rwork | 0.223 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8a4a |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.540 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.097 | |
| Rpim | 0.038 | 0.568 |
| Number of reflections | 27424 | 2719 |
| <I/σ(I)> | 15.26 | 1.28 |
| Completeness [%] | 99.8 | 99.49 |
| Redundancy | 6.8 | 7.1 |
| CC(1/2) | 0.999 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Bis-Tris pH 8.5, 0.2 M KNa-tartrate and 20% (w/v) PEG 3350 |
