8A4A
Structure of human Rep15 in complex with bovine Rab3C.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.977930 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 58.290, 108.795, 172.027 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.970 - 2.520 |
R-factor | 0.2093 |
Rwork | 0.207 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rab |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.970 | 2.610 |
High resolution limit [Å] | 2.520 | 2.520 |
Rmerge | 0.110 | 1.365 |
Rmeas | 0.112 | |
Rpim | 0.031 | 0.386 |
Number of reflections | 18879 | 1827 |
<I/σ(I)> | 18.83 | 2.05 |
Completeness [%] | 99.2 | 99.19 |
Redundancy | 13.3 | 13.2 |
CC(1/2) | 0.999 | 0.713 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0 10% w/v PEG 6000 |