8A3U

Crystal structure of a chimeric LOV-Histidine kinase SB2F1 (symmetrical variant, trigonal form with short c-axis)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97916
Spacegroup name	P 32 2 1
Unit cell lengths	138.716, 138.716, 49.346
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.650 - 2.330
R-factor	0.2341
Rwork	0.232
R-free	0.27970
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	0.819
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	PHENIX (1.19rc6_4061)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.650	2.510
High resolution limit [Å]	2.330	2.330
Rmerge	0.117	2.260
Rmeas	0.120
Rpim	0.027	0.549
Number of reflections	13863	693
<I/σ(I)>	19.8	1.5
Completeness [%]	58.6	14.6
Redundancy	20
CC(1/2)	0.999	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292.15	10% PEG8K, 0.1M Citrate, 0.2M NaCl, 0.1M Ammonium Bromide, 1mM ATP, 2mM MgCl2