8A3P

Structure of human Fy-5.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-19
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	C 2 2 21
Unit cell lengths	94.619, 99.573, 62.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.309 - 2.700
R-factor	0.2247
Rwork	0.220
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u9c
RMSD bond length	0.006
RMSD bond angle	0.894
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.310	47.310	2.850
High resolution limit [Å]	2.700	8.540	2.700
Rmerge		0.047	0.603
Rmeas	0.094	0.050	0.636
Rpim	0.031	0.017	0.201
Total number of observations	79865	2526	12118
Number of reflections	8339	303	1205
<I/σ(I)>	13.4	33.6	3.2
Completeness [%]	99.6	99.4	99.9
Redundancy	9.6	8.3	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES, pH 5.0, 0.8 M Ammonium sulfate