8A3O

Structure of human Fy-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-19
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	P 1 21 1
Unit cell lengths	86.421, 45.273, 93.956
Unit cell angles	90.00, 109.93, 90.00

Refinement procedure

Resolution	44.165 - 2.900
R-factor	0.2418
Rwork	0.237
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yb5
RMSD bond length	0.004
RMSD bond angle	0.709
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.273	45.273	3.060
High resolution limit [Å]	2.900	9.170	2.900
Rmerge		0.052	0.209
Rmeas	0.064	0.059	0.234
Rpim	0.029	0.028	0.103
Total number of observations	74872	2409	11317
Number of reflections	15389	540	2247
<I/σ(I)>	17.9	31.4	6.2
Completeness [%]	99.1	99.4	99.7
Redundancy	4.9	4.5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES pH 6.0, 5 % (w/v) PEG 3000 and 30 % (w/v) PEG 200