8A3B
Cryo-EM structure of mouse Pannexin 1 purified in Salipro nanoparticles
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | TFS Selectris X |
Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.1 |
Number of Particle | 1314251 |
Software | RELION,REFMAC,ISOLDE,EPU,CTFFIND,UCSF ChimeraX |
Symmetry | C7 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |