8A38

RING domain of human TRIM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9766
Spacegroup name	P 32 2 1
Unit cell lengths	48.710, 48.710, 221.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.870 - 2.196
R-factor	0.2592
Rwork	0.258
R-free	0.28810
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.784
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	XDS (Jan 10, 2022)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.870	2.275
High resolution limit [Å]	2.196	2.196
Number of reflections	16478	1574
<I/σ(I)>	22.02
Completeness [%]	99.8	98.5
Redundancy	18.5
CC(1/2)	1.000	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293.15	0.2 M ammonium chloride, 0.1 M sodium acetate, 20% (w/v) PEG 6000, pH 5.0