8A30
Malonyl-CoA reductase from Chloroflexus aurantiacus - C-terminal Apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97626 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.138, 140.181, 74.026 |
Unit cell angles | 90.00, 98.65, 90.00 |
Refinement procedure
Resolution | 55.500 - 1.450 |
R-factor | 0.1775 |
Rwork | 0.176 |
R-free | 0.20100 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.762 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | SHELXD |
Refinement software | REFMAC ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.500 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.041 | 1.441 |
Rmeas | 0.058 | |
Rpim | 0.041 | |
Number of reflections | 154410 | 15025 |
<I/σ(I)> | 10.6 | |
Completeness [%] | 99.4 | |
Redundancy | 1.9 | 1.8 |
CC(1/2) | 0.998 | 0.203 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.8 M Succinic acid, pH 7.0 |