8A2W
Room temperature structure of the ground state of AtPhot2LOV2 in space group P212121, as recovered 1620 seconds after light irradiation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2022-02-02 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.356, 43.191, 133.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.510 - 2.040 |
| R-factor | 0.1818 |
| Rwork | 0.180 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qqk |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.510 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rpim | 0.089 | 0.569 |
| Number of reflections | 10070 | 504 |
| <I/σ(I)> | 5.3 | 1.6 |
| Completeness [%] | 78.0 | |
| Redundancy | 4 | |
| CC(1/2) | 0.980 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM MES pH 6.0, 4 to 9 % PEG8000, and 50 to 200 mM calcium acetate |
