8A2V
Room temperature structure of the ground state of AtPhot2LOV2 in space group P43212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2022-02-02 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 40.829, 40.829, 132.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.010 - 1.590 |
Rwork | 0.165 |
R-free | 0.19960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qqk |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.780 | 1.640 |
High resolution limit [Å] | 1.580 | 1.590 |
Rpim | 0.071 | 0.537 |
Number of reflections | 14046 | 297 |
<I/σ(I)> | 6.3 | 1.3 |
Completeness [%] | 92.1 | |
Redundancy | 8 | |
CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 100 mM MES pH 6.0, 4 to 9 % PEG8000, and 50 to 200 mM calcium acetate |