8A2T
Cryo-EM structure of F-actin in the Mg2+-ADP nucleotide state.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.24 |
Number of Particle | 1296776 |
Software | crYOLO,RELION,PHENIX,EPU,CTFFIND,UCSF ChimeraX,SPHIRE |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |