8A2L

X-ray structure of TRIL-encapsulated human heavy chain ferritin

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0001
Spacegroup name	F 4 3 2
Unit cell lengths	183.060, 183.060, 183.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.690 - 2.301
Rwork	0.171
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n27
RMSD bond length	0.009
RMSD bond angle	1.422
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.690	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.200	1.800
Rmeas		1.000
Rpim	0.059	0.371
Number of reflections	12183	607
<I/σ(I)>	0.711
Completeness [%]	99.9	100
Redundancy	20	15
CC(1/2)	0.998	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	2.0 M magnesium chloride, 0.1 M bicine buffer pH 9.0