8A2G
Crystal structure of Sebokelevirus 2A2 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.350, 69.730, 57.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.270 - 1.560 |
| Rwork | 0.170 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8a2f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.486 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.300 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.055 | 0.726 |
| Number of reflections | 37407 | 2713 |
| <I/σ(I)> | 26.3 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.9 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350, 0.2M sodium fluoride (according to info sent to us) or 30% PEG4000, 0.005 M magnesium acetate, 0.050 M sodium cacodylate pH 6.5 (thesis info) |
