8A1H
Bacterial 6-4 photolyase from Vibrio cholerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 199.687, 199.687, 76.996 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.590 - 1.650 |
R-factor | 0.1194 |
Rwork | 0.119 |
R-free | 0.13620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dja |
RMSD bond length | 0.017 |
RMSD bond angle | 1.365 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.600 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.061 | 0.740 |
Rmeas | 0.064 | 0.785 |
Rpim | 0.019 | 0.261 |
Number of reflections | 108011 | 10661 |
<I/σ(I)> | 25.34 | 2.73 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11 | 8.7 |
CC(1/2) | 1.000 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1 M Tris pH 8.5, 1.5 ammonium sulfate and 12% (v/v) glycerol, 6.25 mg/ml Vc6-4 |