8A0J
Crystal structure of the kinetoplastid kinetochore protein Trypanosoma congolense KKT2 divergent polo-box domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-27 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.700, 57.133, 83.671 |
Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
Resolution | 48.240 - 2.200 |
R-factor | 0.1968 |
Rwork | 0.194 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | alphafold model |
Data reduction software | XDS (Feb 5, 2021) |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.260 | 1.954 |
High resolution limit [Å] | 1.921 | 1.921 |
Number of reflections | 33539 | 1736 |
<I/σ(I)> | 12.3 | |
Completeness [%] | 95.8 | |
Redundancy | 6.8 | |
CC(1/2) | 0.999 | 0.404 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277.15 | 0.1 M HEPES, pH 7.0, 8% w/v Polyvinyl alcohol, 10% v/v 1-Propanol |