8ZYE

Crystal structure of CD38 in complex with RP02 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	80
Detector technology	CCD
Collection date	2022-06-22
Detector	Bruker Platinum 135
Wavelength(s)	0.9785
Spacegroup name	I 2 2 2
Unit cell lengths	47.793, 96.220, 242.459
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.110 - 2.720
R-factor	0.2196
Rwork	0.214
R-free	0.27070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.698
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.110	2.870
High resolution limit [Å]	2.720	2.720
Number of reflections	54357	8096
<I/σ(I)>	10.3
Completeness [%]	90.0
Redundancy	1
CC(1/2)	0.996	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Bis-Tris, pH 6.5, 0.2 M lithium sulfate, and 25% PEG3350