8ZX8
Structure-Based Mechanism and Specificity of Human Galactosyltransferase B3GalT5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-06-17 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.802, 86.535, 87.635 |
| Unit cell angles | 90.00, 95.71, 90.00 |
Refinement procedure
| Resolution | 30.720 - 2.400 |
| R-factor | 0.1998 |
| Rwork | 0.199 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.198 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.742 |
| Number of reflections | 24050 | 1042 |
| <I/σ(I)> | 9.85 | |
| Completeness [%] | 98.0 | 85 |
| Redundancy | 3.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 0.1M BIS-TRIS Propane pH8.5, 0.2M Potassium Sodium Tartrate, 20% w/v PEG 3350 |
