8ZVL
human citrate synthases complexed with oxaloacetate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.600, 78.010, 103.120 |
| Unit cell angles | 70.75, 81.37, 74.74 |
Refinement procedure
| Resolution | 32.680 - 2.050 |
| R-factor | 0.19669 |
| Rwork | 0.194 |
| R-free | 0.25837 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.777 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.680 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.151 | 0.828 |
| Rmeas | 0.181 | 1.012 |
| Number of reflections | 98576 | 7191 |
| <I/σ(I)> | 5.7 | 1.8 |
| Completeness [%] | 96.4 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.990 | 0.423 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M ammonium acetate pH=7.1, 20% w/v PEG3350 |
