Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.060, 167.490, 168.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.330 - 2.600 |
| R-factor | 0.2464 |
| Rwork | 0.244 |
| R-free | 0.28950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hgd |
| RMSD bond angle | 19.056 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.330 | 11.620 | 2.670 |
| High resolution limit [Å] | 2.600 | 8.220 | 2.600 |
| Rmerge | 0.107 | 0.036 | 1.181 |
| Rmeas | 0.111 | 0.038 | 1.230 |
| Number of reflections | 42070 | 931 | 3020 |
| <I/σ(I)> | 18.43 | ||
| Completeness [%] | 99.9 | ||
| Redundancy | 14.4 | ||
| CC(1/2) | 0.999 | 0.999 | 0.845 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 20% PEG8000, 0.1 M HEPES 7.5 |
