8ZUL

Crystal Structure of Human Myt1 Kinase domain Bounded with compound 8m

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-14
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	99.801, 55.834, 58.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.727 - 1.800
R-factor	0.187966331096
Rwork	0.186
R-free	0.21916
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p1a
RMSD bond length	0.011
RMSD bond angle	1.213
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	1.865
High resolution limit [Å]	1.800	1.800
Number of reflections	31010	31010
<I/σ(I)>	2.8
Completeness [%]	99.8
Redundancy	8.7
CC(1/2)	0.488	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	0.8 M Ammonium sulfate,100 mM Sodium citrate,pH 5.5