8ZTC

Crystal structure of Mps1-AMP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	76.733, 76.733, 162.689
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.220 - 2.260
R-factor	0.1989
Rwork	0.196
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.612
Data reduction software	autoPX
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.260	2.260
Rmerge	0.084	0.718
Number of reflections	26616	1942
<I/σ(I)>	44.54	5.14
Completeness [%]	99.5
Redundancy	18.73
CC(1/2)	0.999	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% (v/v) Tacsimate, pH 6.0, 0.05 M sodium cacodylate trihydrate, pH 6.5, and 1.0 mM Spermine