8ZRS

Crystal structure of Skd3, AAA+ only

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-05-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	140.857, 172.407, 140.866
Unit cell angles	90.00, 120.00, 90.00

Refinement procedure

Resolution	14.904 - 3.390
R-factor	0.2307
Rwork	0.229
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.420
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.460
High resolution limit [Å]	3.390	9.210	3.400
Rmerge	0.112	0.045	0.614
Rmeas	0.122	0.048	0.669
Rpim	0.046	0.018	0.261
Number of reflections	79613	4096	3862
<I/σ(I)>	5.2
Completeness [%]	98.9	98.6	96.2
Redundancy	6.8	7.2	6.1
CC(1/2)	1.000	0.998	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	8% PEG 3350, 100 mM HEPES pH 7.5, 100 mM ammonium sulfate