8ZQ3

Structure of ORP1L-RAB7A in the presence of GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-24
Detector	STFC Large Pixel Detector
Wavelength(s)	0.9791
Spacegroup name	P 41 2 2
Unit cell lengths	80.420, 80.420, 134.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.210 - 2.430
R-factor	0.1978
Rwork	0.194
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t91
RMSD bond length	0.008
RMSD bond angle	1.016
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.720	2.500
High resolution limit [Å]	2.430	2.430
Number of reflections	17217	17217
<I/σ(I)>	17.8
Completeness [%]	99.8
Redundancy	25.6
CC(1/2)	0.999	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M diammonium tartrate, 20% PEG3350, 0.1 M Ethylenediaminetetraacetic acid disodium salt dihydrate