8ZN9
A vast marine sulfonate-based carbon cycle fueled by novel sulfoquinovosidases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.920, 56.690, 217.300 |
| Unit cell angles | 90.00, 96.10, 90.00 |
Refinement procedure
| Resolution | 30.480 - 1.800 |
| R-factor | 0.2198 |
| Rwork | 0.219 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.813 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.480 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 232860 | 232860 |
| <I/σ(I)> | 11.01 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.2 | 0.1 M Ammonium acetate, 0.1 M BIS-TRIS pH 5.5, 16 % PEG 10000 |
