8ZMN

Crystal structure of ANTXR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	38.516, 39.224, 102.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.640 - 2.140
R-factor	0.2126
Rwork	0.210
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.748
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.640	2.217
High resolution limit [Å]	2.140	2.140
Rmerge	0.154	1.007
Number of reflections	7809	5973
<I/σ(I)>	9.96
Completeness [%]	86.0	98.38
Redundancy	8.3	7
CC(1/2)	0.996	0.693

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.2 M Sodium chloride, 0.1 M BIS-TRIS pH 6.5, 28% w/v Polyethylene glycol 3,350