8ZJV
Crystal Structure of the ERK2 complexed with 5-Iodotubercidin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-03-28 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 96.979, 96.979, 95.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.520 - 1.800 |
R-factor | 0.1915 |
Rwork | 0.190 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ax3 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.798 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.520 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.128 | 2.026 |
Number of reflections | 48367 | 4796 |
<I/σ(I)> | 16.67 | |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 15.1 | 15.5 |
CC(1/2) | 0.999 | 0.685 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.2 M Lithium Sulfate monohydrate, 0.1 M Bis-Tris pH 5.5, 25% w/v Polyethylene Glycol 3350 |