8ZIG

Crystal structure of the methyltransferase PsiM in complex with s-adenosylmethionine (SAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	58.339, 126.744, 39.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.700 - 1.350
R-factor	0.1836
Rwork	0.182
R-free	0.20380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8zie
RMSD bond length	0.006
RMSD bond angle	1.020
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.370	1.430
High resolution limit [Å]	1.350	1.350
Rmerge	0.074	0.593
Rmeas	0.084	0.694
Rpim	0.028	0.307
Number of reflections	60331	5949
<I/σ(I)>	16.4	2.7
Completeness [%]	93.1	64.2
Redundancy	8.6	4.7
CC(1/2)	0.999	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene glycol 4,000