8ZH5

The Crystal Structure of the ROCK1 from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-07
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 43 21 2
Unit cell lengths	166.283, 166.283, 64.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.161 - 3.700
Rwork	0.208
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.790
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	4.050
High resolution limit [Å]	3.700	3.700
Rmerge	0.235	0.917
Number of reflections	9205	2184
<I/σ(I)>	11.9
Completeness [%]	91.4
Redundancy	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MgAC2, 0.1M NaAC pH4.5, 8% PEG 8000, 1.0M NDSB-256