8ZGD
The Crystal Structure of the NLRP1_LRR domain from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-01-27 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.952989 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 84.582, 84.582, 132.495 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.130 - 2.050 |
Rwork | 0.176 |
R-free | 0.22090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.929 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.130 | 2.110 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.210 | 0.964 |
Number of reflections | 18288 | 1387 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 100.0 | |
Redundancy | 19.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20mM MgCl2, 0.1M Tris pH 8.0, 19% polyacrylic acid 5100 |