8ZG8

ZZ-domain of the Arabidopsis thaliana E3 ubiquitin-protein ligase PRT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.28
Spacegroup name	P 62 2 2
Unit cell lengths	78.030, 78.030, 70.452
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.770 - 1.744
R-factor	0.2392
Rwork	0.235
R-free	0.27860
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.731
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.770	1.806
High resolution limit [Å]	1.744	1.744
Rmerge	0.084	2.158
Rmeas	0.086	2.212
Rpim	0.018	0.477
Number of reflections	13080	1197
<I/σ(I)>	32.13	1.37
Completeness [%]	97.2	90.92
Redundancy	21.4	20.5
CC(1/2)	1.000	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	4 M sodium formate